Education

PHD, 1995, University of Hawaii, Manoa, Honolulu
M.S., 1981, Punjab Agricultural University
BSC, 1978, University of Delhi

Languages

Hindi
Punabi
Urdu

Relevant Publications

Parveen, N., Fernandez, M.C., Haynes, A.M., Zhang, R.L., Godornes, B.C., Centurion-Lara ,A., Giacani L. 2019. Non-pathogenic Borrelia burgdorferi expressing Treponema pallidum TprK and Tp0435 antigens as a novel approach to evaluate syphilis vaccine candidates. Feb 20. pii: S0264-410X(19)30217-8. doi: 10.1016/j.vaccine.2019.02.022

Djokic, V., Primus, S., Akoolo, L., Chakraborti, M. and N. Parveen. 2018. Age-related differential stimulation of immune response by Babesia microti and Borrelia burgdorferi during acute phase of infection affect diseases severity. Frontiers in Immunology. 7;9:2891. doi: 10.3389/fimmu.2018.02891

Bhanot, P., and N. Parveen. 2018. Severity of symptoms of babesiosis alone or with Lyme disease in the mouse infection model. Invited review. International Journal of Parasitology. International Journal of Parasitology. 49(2):145-151. doi: 10.1016/j.ijpara.2018.06.006.

Primus, S., Akoolo, L., Schlachter, S., N. Parveen. 2018. Efficient detection of symptomatic and asymptomatic patient samples for Babesia microti and Borrelia burgdorferi infection by multiplex qPCR. PLoS One. 10;13(5):e0196748. doi: 10.1371/journal.pone.0196748.

Djokic, V., Akoolo, L., and N. Parveen. 2018. Babesia microti Infection Changes Host Spleen Architecture and Is Cleared by a Th1 Immune Response. Frontiers in Microbiology. 9:85. doi: 10.3389/fmicb.2018.00085. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797759

Primus, S., Akoolo, L., Schlachter, S., N. Parveen. 2018. Screening of patient blood samples for babesiosis using enzymatic assays. Tick and Tick Borne Diseases. 9(2):302- 306. doi:10.1016/j.ttbdis.2017.11.003

Schlachter, S., Seshu, J., Lin, T., Norris, S., N. Parveen. 2018. Borrelia burgdorferi glycosaminoglycan binding protein, Bgp in the B31 strain is not essential for infectivity despite facilitating adherence and tissue colonization. Infection and Immunity. 22;86(2). pii: e00667-17. doi: 10.1128/IAI.00667-17

Akoolo, L., Schlachter, S., Khan, K., Alter, L., Rojtman , A. D., Gedroic, K., Bhanot, P., and N. Parveen. 2017. A novel quantitative PCR detects Babesia infection in patients not identified by currently available non-nucleic acid amplification tests. BMC Microbiology 17:16-24. doi:10.1186/s12866-017-0929-2

Chan, K., Nasereddin, T., Alter, L., Centurion-Lara, A., Giacani,G., and N. Parveen. 2016. "Treponema pallidum Lipoprotein TP0435 Expressed in Borrelia burgdorferi Produces Multiple Surface/Periplasmic Isoforms and mediates Adherence". Nature Scientific Reports 6:25593. doi:10.1038/srep25593.

Areas Of Interest

Borrelia burgdorferi

Lyme disease

Pseudomonas aeruginosa

Treponema pallidum

bacterial-host interactions

diagnostic tests

innovative approach and surrogate system

molecular mechanisms of pathogenesis

novel drug targets

proteases

pyocyanin; infectious diseases

syphilis

Course List

EDUC7001K
TIII5021Q		MolecPathogen of Bacteria
TIII5155Q
TIII5620Q		Classic/21st Cent Pathogens

Virulence Factors Of Pseudomonas Aeruginosa And Lyme Disease Spirochete, Borrelia Burgdorferi

My laboratory is studying the molecular basis of pathogenesis of bacterial species, Borrelia burgdorferi, Treponema pallidum and Pseudomonas aeruginosa. These clinically important bacterial pathogens are transmitted to humans using different mechanisms and also show different disease manifestations. B. burgdorferi is transmitted by Ixodes tick vector, T. pallidum by sexual contact and P. aeruginosa, a ubiquitously present organism, is transmitted through ventilation or by direct contact of the patient with the contaminated source.

B. burgdorferi, a spirochete, is causative agent of Lyme disease, a multisystemic illness that affects various organs including joints, heart, nervous system and skin. If untreated, parveen_figure2_2010.jpgit may result in chronic disease with the symptoms including arthritis, acrodermatitis or neuroborreliosis. It is an extracellular pathogen often found adhering to the host cells in the biopsy specimens of the patients. We have been studying the molecular mechanisms involved in the attachment of Lyme disease spirochetes to a variety of host cells. The specific interaction between the spirochete and host cells may be responsible for the tissue tropism exhibited by B. burgdorferi. Our objective is to understand whether different B.burgdorferi adhesins show affinity for different host receptors on various host cells. We use genetics, biochemical techniques and tissue culture system to identify and characterize the bacterial and host molecules involved in this interaction in vitro. We have already identified two types of glycosaminoglycan receptors on mammalian cells that are recognized by several B. burgdorferi proteins and we are further characterizing this interaction. Mouse is a natural host of B. burgdorferi and C3H mice show several manifestations of Lyme disease observed in humans. We have recently adapted firefly luciferase-based detection system for B. burgdorferi. Using a combination of bioluminescent B. burgdorferi and mouse model of infection, we will further analyze the contribution of each bacterial ligand-host receptor interaction in Lyme pathogenesis. Tissue colonization by the spirochetes will be monitored non-invasively by employing in vivo imaging system. Recently, we have initiated studies to understand molecular basis of T. pallidum pathogenesis using this as a surrogate system.


P. aeruginosa is an opportunistic pathogen and produces a wide variety of virulence factors. parveen_figure2010.jpgIt results in a variety of illnesses and is responsible for high morbidity and mortality in immunocompromised and elderly patients. Due to a highly adaptable nature of P. aeruginosa and its ability to survive even in detergents, it is a major contributor to infections in the hospital environment. We have been studying the quorum-sensing mediated induction of several virulence factors in this organism both as free-living organism and in association with its different hosts. We will assess the role of selected virulence factors in biofilm formation while P. aeruginosa is present in communities along with the other organisms. Our current focus is to investigate genetics of production and regulation of PrpL protease and pyocyanin pigment of P. aeruginosa and examine the roles of these virulence factors in tissue destruction. The roles of these two virulence factors in corneal damage, in burn wounds and in the cystic fibrosis patients will then be examined.